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Xpress
Custom Microarray Technology
Xpress Custom Microarray Technology
(CMT)
is a complete set of protocols for all steps of microarrays fabrication, processing and analysis.
Xpress™
CMT addresses the most critical demands of researchers deal with
specificity of experiments, repeatability and reliability of
experimental data. Due to low requirements for DNA and RNA
material Xpress™
CMT brings microarrays experiments to the new quality
level and makes them suitable for diagnostics and medical
applications.
0.75 ug of amplified DNA material of each clone is enough for printing microarrays
0.5 ug of Poly-A RNA is enough for hybridization of one microarray
HIGHLIGHTS
- Saves and reuses precious biological material.
- Provides excellent spotting quality
- Reaches highest results in sensitivity, specificity and repeatability of the assays.
- Minimizes requirements for prior experience of the lab personnel.
Xpress CMT requires 6-10 times less of DNA and RNA amounts for microarray experiments compare to other published methods. Due to extremely low requirements for biological material Xpress CMT is ideally suited for diagnostic and
medical applications.
The printing buffer specified in Xpress CMT allows long-term storage of dissolved DNA clones at –18C for further prints.
Xpress Line CMT utilizes the reduced cycle of post print processing by elimination
of all high temperature processing steps. This approach significantly lowers background signal of the microarray substrate surface.
Xpress CMT includes complete specifications of the compounds and components used for microarray processing. All chemical components are common to any lab and available from multiple vendors.
Although the Xpress CMT is best optimized for using with LabNEXT equipment it is possible to use it with equipment from other vendors.
Xpress CMT Artifacts:
Xpress CMT is a step-by-step guide for microarray processing delivered in hard copy or electronic document format. It covers all stages from printing preparations to interpretation of experimental data. The description comes along with specifications of all chemical solutions required for microarray processing. LabNEXT does not provide chemical solutions because all of them consist of commonly used compounds available in any biological lab and can be easily prepared according to the protocols provided.
Xpress CMT includes the following:
- Unique microarray spotting buffer and microarray production protocols
- Protocols for post-print microarray processing.
- Hybridization technique protocols and compounds
- Quality control procedures
- Complete catalogue of compounds and materials used for microarray operations.
Xpress
CMT Features
Excellent Spotting Uniformity
The unique spotting buffer provides perfect spotting uniformity even for DNA solutions with different physical parameters. It keeps spot’s size and shape in broad range of environmental conditions such as humidity and temperature and makes unnecessary bulky and expensive humidifiers, dehumidifiers and temperature control units.
Fig
#3. Fragment of diagnostic microarray image manufactured with Xpress CMT and Lab
Next equipment.
90% DNA binding
Printing microarrays in the Lab Next spotting buffer allows retaining over 90% of DNA on the glass surface available for hybridization. This makes possible to reduce amount of amplified material to 0.75 ug per DNA clone.
Alternative methods that were published by Incyte Genomics or being recommended by TeleChem International require up to 8 ug of amplified DNA product.
Experimental verification of DNA binding efficiency in different printing solutions:
TeleChem Microspotting Buffer,0.5M NaSCN, 1M AmAc, 2x SSC (Incyte Genomics),
LabNEXT printing Buffer, TE.
Fig.
#1 Microarray printed with pre-labeled DNA scanned right after printing.
Horizontal lines of The spots correspond to different spotting buffers.
Fig.
#2 The same microarray scanned after the post printing treatment. Two lines of
the spots (green) correspond to the LabNEXT spotting buffer. These spots
retained more labeled DNA molecules and produced higher fluorescence signal.
DNA labeling
efficiency
Conditions of the labeling reaction were chosen to reach the maximal inclusion of fluorescent label in cDNA molecules. Highly efficient labeling allows using of exceptionally small amount of Poly-A RNA (0.5 ug) for probe preparation. This makes possible using of the microarrays in experiments with clinical samples where RNA availability is usually limited.
100% hybridization efficiency
Xpress CMT hybridization technique allows 100% of labeled molecules to participate in the reaction. This significantly reduces chances of non-specific binding and lowers fluorescent background of the area around the spots.
Xpress CMT offers methods of setting multiple hybridization reactions on a single microarray substrate leveraging advantages of identical hybridization conditions for separate experiments. (This feature requires multi-zone microarrays printed with Xact Personal
Microarrayer)
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